Really odd question
Jun. 24th, 2006 01:15 pm![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png){kind=small}
jebbypalOkay, if anyone has any friends on their flists that are in research or just happen to know a lot about gene expression, I'd really appreciate it if you direct them here. My specialty is immunology even if my project is control of gene expression and I'm far from an expert on this but the question is driving me nuts and I can't think of anyone RL to ask about it yet.
Okay, so here's the deal --- I'm looking at the expression of a gene and all the promoter studies have been done via LPS, PMA, or CD3 stimulation. However, it is known that a cytokine can upregulate it's expression and is debated whether the cytokine actually induces expression on it's own (arguments being ELISA doesn't detect, RNA depends on which cell type you look at, and possibilities of endotoxin/lps contamination). However, it does act synergistically with LPS to increase expression.
However, by luciferase studies, the cytokine can't drive luciferase expression through any combo of the expressed genes promoter or intronic sequences. I do know that cytokine treatment causes alteration in histone modifications.
Soo, are there genes in which you can drive gene expression without being able to get a positive result from a luciferase assay?
Okay, so here's the deal --- I'm looking at the expression of a gene and all the promoter studies have been done via LPS, PMA, or CD3 stimulation. However, it is known that a cytokine can upregulate it's expression and is debated whether the cytokine actually induces expression on it's own (arguments being ELISA doesn't detect, RNA depends on which cell type you look at, and possibilities of endotoxin/lps contamination). However, it does act synergistically with LPS to increase expression.
However, by luciferase studies, the cytokine can't drive luciferase expression through any combo of the expressed genes promoter or intronic sequences. I do know that cytokine treatment causes alteration in histone modifications.
Soo, are there genes in which you can drive gene expression without being able to get a positive result from a luciferase assay?